Table 4 Sequences of the oligonucleotide primers used in this study
Primer names | Sequence (5′–3′), 15-bp homologous sequence for infusion is underlined |
|---|---|
F1 | GTTCTCCAGAAGCGACATGGACATATTAATGACATTGTCATCAACG |
R1 | CACAGACACCCTAGGCTAGTTAAGGCACTGGGCGTAGTAGGG |
F2 | GTTCTCCAGAAGCGACAGACCATCCAGCCCGGCACC |
R2 | CACAGACACCCTAGGTTAGCTCACGGTGATAGAGGCAGAGCCA |
JMP1F | CCTAGGGTGTCTGTGGTATCTAAGCTATT |
JMP1R | TCGCTTCTGGAGAACTGCGG |
F3 | ACACCCGAAGGATCCCACAATGAAGCTTTCCACCATCC |
R3 | ATGGTGATGATGGTGGCTCACGGTGATAGAGGCAGAG |
R4 | ATGGTGATGATGGTGGTTAAGGCACTGGGCGTAGTAGGG |
R5 | ATGGTGATGATGGTGGTTCTGGGAAAACTGGACGCC |
JMP2F | CACCATCATCACCATCATTAAAACT |
JMP2R | GGATCCTTCGGGTGTGAGTTG |
HphF | CCACACACATCCACAATGAAAAAGCCTGAACTCACCGCGA |
HphR | TAGCAGGGCAGGGCCCTATTCCTTTGCCCTCGGACGAGTG |
LHFF | GGCCCTGCCCTGCTAATGAAATG |
LHFR | TGTGGATGTGTGTGGTTGTATGTGTGATG |
XLF | GCTCTAGACGATGCCGCCGCAAGGAATG (XbaI) |
XLR | CGTCTAGATGGAATTCGATTTGTCTTAGAGGAACGCA (XbaI) |