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Table 3 Primers used in this work

From: Lipopeptide produced from Bacillus sp. W112 improves the hydrolysis of lignocellulose by specifically reducing non-productive binding of cellulases with and without CBMs

Type

Sequence (from 5′ to 3′)

Restriction enzyme

45818-WT

Forward

CGCGGATCCACACTGCAGTCTAATCTGGTTGTAA

BamHÓ€

Reverse

CCGCTCGAGTTAAATTTCTGTTCCGCCGTAAGTA

XhoÓ€

45818-Core

Forward

CGCGGATCCAAAGAGGAAAATGACATCGTTCC

BamHÓ€

Reverse

CCGCTCGAGATGAGTTACTTTAATGCCATTTGTA

XhoÓ€