Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution

Matsusako, Takuya; Toya, Yoshihiro; Yoshikawa, Katsunori; Shimizu, Hiroshi

doi:10.1186/s13068-017-0996-5

Biotechnology for Biofuels and Bioproducts

Table 1 Details about the mutations of evolved strains

From: Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution

Strain	Type^a	Position	Reference	Mutation	Gene	Type	Mutation ratio (%)^b	Function
T(1)	Del	492349	ATCCTA	-T----	slr1044 (mcpA)	Frameshift, stop codon	95	Methyl-accepting chemotaxis protein
T(1)	Ins	2364114	CCG	CCGA	slr0369 (envD)	Frameshift, stop codon	94	RND multidrug efflux transporter
T(2)	Ins	345787	TGG	TGGG	sll0923 (epsB)	Frameshift, stop codon	92	Unknown protein
	Ins	389860	CCC	CCCC	sll1025	Frameshift, stop codon	86	Hypothetical protein
	Ins	549361	CGG	CGGG	sll1636 (fbp)	Frameshift, stop codon	84	Ferripyochelin-binding protein
	SNP	755131	CTG	TTG	sll1924 (sycrp2)	Leu→Leu	99	cAMP receptor protein
	Ins	1041211	GGG	GGGG	slr1233 (frdA)	Frameshift, stop codon	94	Succinate dehydrogenase flavoprotein subunit
	Del	2270044	GGG	-GG	slr0322 (hik43)	Frameshift, stop codon	93	Two-component hybrid sensor and regulator
	SNP	2279149	CAG	GAG	slr0326	Gln→Glu	97	Hypothetical protein
	SNP	2364333	GTT	GGT	envD	Val→Gly	100	RND multidrug efflux transporter
	Del	2585782	CCC	-CC	sll0058 (dnaK)	Frameshift, stop codon	93	Heat-shock protein 70, molecular chaperone
	Del	3430453	CTT	C-T	slr0756 (kaiA)	Frameshift, stop codon	95	Circadian clock protein KaiA homolog
	Ins	1372331	CAA	CAAC	sll1165 (mutS)	Frameshift, stop codon	80	DNA mismatch repair protein MutS
T(3)	Del	1766850	ACCGCCCTTCCAACG	AC------------G	slr1963 (ocp)	Del (Ala, Leu, Pro)	88	Water-soluble carotenoid protein
	SNP	2271565	TGG	TGA	hik43	Stop codon	98	Two-component hybrid sensor and regulator
	Del	2366401	TCGGGGCGA	---------	envD	Del (Ser, Gly, Arg)	88	RND multidrug efflux transporter
T(4)	Ins	492262	TAT	TATT	mcpA	Frameshift, stop codon	95	Methyl-accepting chemotaxis protein
T(4)	SNP	2366659	GTT	TTT	envD	Val→Phe	99	RND multidrug efflux transporter

^aDel, Ins, and SNP indicate an deletion, insertion, and single nucleotide polymorphism, respectively
^bMutation ratios indicates

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