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Table 1 Details about the mutations of evolved strains

From: Identification of alcohol stress tolerance genes of Synechocystis sp. PCC 6803 using adaptive laboratory evolution

Strain Typea Position Reference Mutation Gene Type Mutation ratio (%)b Function
T(1) Del 492349 ATCCTA -T---- slr1044 (mcpA) Frameshift, stop codon 95 Methyl-accepting chemotaxis protein
Ins 2364114 CCG CCGA slr0369 (envD) Frameshift, stop codon 94 RND multidrug efflux transporter
T(2) Ins 345787 TGG TGGG sll0923 (epsB) Frameshift, stop codon 92 Unknown protein
Ins 389860 CCC CCCC sll1025 Frameshift, stop codon 86 Hypothetical protein
Ins 549361 CGG CGGG sll1636 (fbp) Frameshift, stop codon 84 Ferripyochelin-binding protein
SNP 755131 CTG TTG sll1924 (sycrp2) Leu→Leu 99 cAMP receptor protein
Ins 1041211 GGG GGGG slr1233 (frdA) Frameshift, stop codon 94 Succinate dehydrogenase flavoprotein subunit
Del 2270044 GGG -GG slr0322 (hik43) Frameshift, stop codon 93 Two-component hybrid sensor and regulator
SNP 2279149 CAG GAG slr0326 Gln→Glu 97 Hypothetical protein
SNP 2364333 GTT GGT envD Val→Gly 100 RND multidrug efflux transporter
Del 2585782 CCC -CC sll0058 (dnaK) Frameshift, stop codon 93 Heat-shock protein 70, molecular chaperone
Del 3430453 CTT C-T slr0756 (kaiA) Frameshift, stop codon 95 Circadian clock protein KaiA homolog
Ins 1372331 CAA CAAC sll1165 (mutS) Frameshift, stop codon 80 DNA mismatch repair protein MutS
T(3) Del 1766850 ACCGCCCTTCCAACG AC------------G slr1963 (ocp) Del (Ala, Leu, Pro) 88 Water-soluble carotenoid protein
SNP 2271565 TGG TGA hik43 Stop codon 98 Two-component hybrid sensor and regulator
Del 2366401 TCGGGGCGA --------- envD Del (Ser, Gly, Arg) 88 RND multidrug efflux transporter
T(4) Ins 492262 TAT TATT mcpA Frameshift, stop codon 95 Methyl-accepting chemotaxis protein
SNP 2366659 GTT TTT envD Val→Phe 99 RND multidrug efflux transporter
  1. aDel, Ins, and SNP indicate an deletion, insertion, and single nucleotide polymorphism, respectively
  2. bMutation ratios indicates