Overexpression of a Domain of Unknown Function 231-containing protein increases O-xylan acetylation and cellulose biosynthesis in Populus

Table 1 List of metabolites with increased content (µg/g FW sorbitol equivalents) in leaf tissues of 6-month-old greenhouse-grown OXPdDUF231A versus K-147 plants

Metabolite [retention time (min); key m/z]	OXPdDUF231A/K-147		OXPdDUF231A		K-147
Metabolite [retention time (min); key m/z]	Fold change	p value	Avg	sem	Avg	sem
19.63 171 Feruloyl-caffeoyl glycoside	#DIV/0!	0.282	0.4	0.3	0	0
17.04 354 Guaiacyl lignan	#DIV/0!	0.094	1.0	0.3	0	0
17.33 354 Syringyl lignan glycoside	#DIV/0!	0.081	1.2	0.4	0	0
18.48 Feruloyl-caffeoyl-shikimate glycoside	59.16	0.083	15.2	5.0	0.3	0.0
13.27 235 Xylopyranoside	30.75	0.001	2685.9	330.7	87.3	15.0
1,5-Dicaffeoyl-shikimate	25.92	0.008	99.0	17.9	3.8	0.3
21.01 557 193 647 Glycoside	15.61	0.252	4.5	2.3	0.3	0.0
13.63 278 309 227	11.67	0.002	7.6	1.0	0.7	0.0
18.43 616 386 Caffeoyl-shikimate glycoside conjugate	9.61	0.243	0.8	0.4	0.1	0.0
Syringin	9.06	0.199	13.7	5.9	1.5	0.1
1,2,3-Benzenetriol	5.42	0.002	15.2	1.8	2.8	0.5
Raffinose	4.75	0.066	86.6	21.4	18.2	4.0
Maleic acid	3.49	0.028	406.5	69.4	116.5	38.4
Erythronic acid	3.46	0.046	42.4	8.5	12.2	1.0
18.11 235 Phenolic glycoside	3.46	0.263	0.6	0.2	0.2	0.0
Salicyl alcohol	3.38	0.020	113.5	18.1	33.5	5.3
Galactinol	3.25	0.160	74.7	22.4	23	4.1
12.81 539 359 320	3.23	0.026	15.1	2.5	4.7	0.3

Metabolites were analyzed as trimethylsilyl derivatives by gas chromatography–mass spectrometry. Data are the average (avg) and standard error of the mean (sem) of six OXPdDUF231A plants (three plants from each of two independent transgenic lines) and three K-147 control plants. Unknown and partially identified metabolites are designated by retention time, key mass-to-charge (m/z) ratios, and identified moieties. #DIV/0! represents an integration area divided by zero (absence)

ISSN: 2731-3654