Table 1 S. cerevisiae strains used in this study

CEN.PK113-5D

MATa ura3-52

–

[29]

CEN.PK122

MATa/MATα

–

[29]

IMX585

MATa URA3 can1::cas9-natNT2

CEN.PK113-7D

[34]

IMX581

MATa ura3-52 can1::cas9-natNT2

CEN.PK113-5D

[34]

IMX673

MATa/MATα ura3-52/ura3-52 CAN1/can1Δ::cas9-natNT2

CEN.PK115

[34]

IME324

MATa ura3-52 can1::cas9-natNT2 p426-TEF (empty)

IMX581

[22]

IMX765^a

MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL

IMX581

This study

IMX773

MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL X-2::pYEN1-prk/X-2::pYEN1-prk pUDR164

IMX765

This study

IMX774

MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL X-2::pDAN1-prk/X-2::pDAN1-prk pUDR164

IMX765

This study

IMX949

MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL X-2::pDAN1-prk/X-2::pDAN1-prk gpd2Δ/gpd2Δ pROS10-gRNA.GPD2

IMX774

This study

IMX1443

MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL X-2::pDAN1-prk/X-2::pDAN1-prk gpd2::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2/gpd2::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2 pUDR164

IMX774

This study

IME369

MATa/MATα ura3-52/ura3-52 CAN1/can1Δ::cas9-natNT2 p426-TEF (empty)

IMX673

This study

IMX1472

MATa ura3-52 can1::cas9-natNT2 gpd2::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2 pROS11-gRNA.GPD2

IMX581

This study

IMX1489

MATa ura3-52 can1::cas9-natNT2 gpd2::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2 sga1::pDAN1-prk, cbbm (9 copies), groES, groEL pUDR103

IMX1472

This study