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Table 1 S. cerevisiae strains used in this study

From: Optimizing anaerobic growth rate and fermentation kinetics in Saccharomyces cerevisiae strains expressing Calvin-cycle enzymes for improved ethanol yield

Strain name Relevant genotype Parental strain Origin
CEN.PK113-5D MATa ura3-52 [29]
CEN.PK122 MATa/MATα [29]
IMX585 MATa URA3 can1::cas9-natNT2 CEN.PK113-7D [34]
IMX581 MATa ura3-52 can1::cas9-natNT2 CEN.PK113-5D [34]
IMX673 MATa/MATα ura3-52/ura3-52 CAN1/can1Δ::cas9-natNT2 CEN.PK115 [34]
IME324 MATa ura3-52 can1::cas9-natNT2 p426-TEF (empty) IMX581 [22]
IMX765a MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL IMX581 This study
IMX773 MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL X-2::pYEN1-prk/X-2::pYEN1-prk pUDR164 IMX765 This study
IMX774 MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL X-2::pDAN1-prk/X-2::pDAN1-prk pUDR164 IMX765 This study
IMX949 MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL X-2::pDAN1-prk/X-2::pDAN1-prk gpd2Δ/gpd2Δ pROS10-gRNA.GPD2 IMX774 This study
IMX1443 MATa/MATa ura3-52/ura3-52 can1::cas9-natNT2/can1::cas9-natNT2 sga1::cbbm (9 copies), groES, groEL/sga1::cbbm (9 copies), groES, groEL X-2::pDAN1-prk/X-2::pDAN1-prk gpd2::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2/gpd2::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2 pUDR164 IMX774 This study
IME369 MATa/MATα ura3-52/ura3-52 CAN1/can1Δ::cas9-natNT2 p426-TEF (empty) IMX673 This study
IMX1472 MATa ura3-52 can1::cas9-natNT2 gpd2::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2 pROS11-gRNA.GPD2 IMX581 This study
IMX1489 MATa ura3-52 can1::cas9-natNT2 gpd2::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2 sga1::pDAN1-prk, cbbm (9 copies), groES, groEL pUDR103 IMX1472 This study
  1. aIndicates spontaneous diploidization; see “Results” section