Table 2 Plasmids used in this study

p426-TEF

2 μm ori, URA3, pTEF1-tCYC1 empty vector

[38]

pMEL10

2 μm ori, KlURA3, pSNR52-gRNA.CAN1-tSUP4

[34]

pMEL11

2 μm ori, amdS, pSNR52-gRNA.CAN1-tSUP4

[34]

pROS10

URA3, gRNA.CAN1-2 μm ori-gRNA.ADE2

[34]

pROS11

amdS, gRNA.CAN1-2 μm ori-gRNA.ADE2

[34]

pUD232

Delivery vector, pTEF1-groEL-tACT1

[28]

pUD233

Delivery vector, pTPI1-groES-tPGI1

[28]

pUDE046

2 μm ori, pGAL1-prk-tCYC1

[28]

pBTWW002

2 μm ori, URA3, pTDH3-cbbm-tCYC1

[28]

pUD344

pPGI1-NQM1-tNQM1 PCR template vector

[48]

pUD345

pTPI1-RKI1-tRKI1 PCR template vector

[48]

pUD346

pPYK1-TKL2-t-TKL2 PCR template vector

[48]

pUD347

pTDH3-RPE1–tRPE1 PCR template vector

[48]

pUD348

pPGK1-TKL1-tTKL1 PCR template vector

[48]

pUD349

pTEF1-TAL1-tTAL1 PCR template vector

[48]

pUDR103

2 μm ori, KlURA3, pSNR52-gRNA.SGA1-tSUP4

[22]

pUDR119

2 μm ori, amdS, pSNR52-gRNA.SGA1-tSUP4

This study

pUDR164

2 μm ori, KlURA3, pSNR52-gRNA.X-2-tSUP4

This study

pJET-cbbm

PCR template vector for cbbm amplification

This study