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Table 4 Yields (Y) of biomass and ethanol on glucose in anaerobic chemostat cultures of S. cerevisiae reference strain IME324 and the RuBisCO/PRK-expressing strain IMX774

From: Optimizing anaerobic growth rate and fermentation kinetics in Saccharomyces cerevisiae strains expressing Calvin-cycle enzymes for improved ethanol yield

Strain IME324 IMX774
Relevant genotype GPD1 GPD2 GPD1 GPD2 pDAN1-prk cbbm
Dilution rate (h−1) 0.05 0.15 0.05 0.15
Y biomass/glucose (g g−1) 0.083 ± 0.001 0.087 ± 0.007 0.082 ± 0.002 0.086 ± 0.002
Y ethanol/glucose (g g−1) 0.421 ± 0.001 0.411 ± 0.006 0.451 ± 0.001*,# 0.432 ± 0.001*,#
  1. Cultures were grown on synthetic medium containing 20 g L−1 glucose (pH 5). Values represent averages ± mean deviations of measurements on independent duplicate cultures. * (p < 0.05) denotes statistical significance of differences between strains IME324 and IMX774 at the same dilution rate and # (p < 0.01) indicates statistical significance of differences between analyses at different dilution rates in cultures of the same strain in Student’s t tests. Degree of reduction balances of steady-state analyses yielded electron recoveries between 99 and 101%
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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