Strain
|
IME369
|
IMX1489
|
---|
Relevant genotype
|
GPD1 GPD2
|
GPD1 gpd2Δ pDAN1-prk
cbbm non-ox PPP↑
|
---|
μ (h−1)
|
0.31 ± 0.00
|
0.30 ± 0.01
|
Y biomass/glucose (g g−1)
|
0.091 ± 0.009
|
0.096 ± 0.001*
|
Y ethanol/glucose (g g−1)
|
0.376 ± 0.005
|
0.421 ± 0.002*
|
Y glycerol/glucose (g g−1)
|
0.107 ± 0.004
|
0.014 ± 0.000**
|
Glycerol produced/biomass [mmol (g biomass)−1]
|
12.189 ± 1.080
|
1.669 ± 0.082**
|
- Cultures were grown on synthetic medium containing 20 g L−1 glucose (pH 5). Specific growth rates and stoichiometries were calculated from sample points during the mid-exponential growth phase. Values represent averages ± mean deviations of measurements on independent duplicate cultures. * (p < 0.02) and ** (p < 0.01) denote statistical significance of differences between IME324 (Table 3) and strains IME369 and IMX1489 in Student’s t tests. Degree of reduction balances constructed over the exponential growth phase yielded electron recoveries between 96 and 100%