Fig. 10

Glycome profiling of P. deltoides PtGAUT12.1-OE lines. Cell walls (AIR) prepared from WT and PtGAUT12.1-OE lines (AB29.2, AB29.7, and AB29.12) were sequentially extracted using increasingly harsh reagents as described in “Methods.” The resulting wall extracts were screened by ELISA using 155 monoclonal antibodies (mAbs) directed against epitopes present on most major non-cellulosic plant cell wall glycans [25, 29]. The binding response data are presented as heatmaps using a white-red-dark-blue scale indicating the strength of the ELISA signal (white, red, and dark-blue colors depict strong, medium, and no binding, respectively). The mAbs are grouped based on the cell wall glycans they predominantly recognize as depicted in the panel on right-hand side of the figure. The gravimetric amounts of materials extracted from the wall by each extracting reagent are shown as bar graphs above the heatmaps. Dotted boxes show major regions of the profiles where increased/reduced antibody binding was observed in PtGAUT12.1-OE wall extracts