Fig. 3From: Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in PopulusPtGAUT12.1 gene model, overexpression vector map, and GAUT12.1 transcript abundance in P. deltoides PtGAUT12-OE lines. A PtGAUT12.1 (Potri.001G416800) gene model from Phytozome 11.0 Populus trichocarpa v3.0. nt, nucleotides. B Structure of PtGAUT12.1 (Potri.001G416800) mRNA. CDS, coding sequence. C A schematic of the PtGAUT12.1 (Potri.001G416800) overexpression construct used to generate P. deltoides PtGAUT12.1 overexpression lines. D Relative GAUT12.1 and GAUT12.2 transcript abundance as determined by quantitative RT-PCR analysis of 3-month-old P. deltoides wild-type (WT) and PtGAUT12.1-OE lines. The 18S rRNA was used as the reference gene and the transcript expression of PdGAUT12.2 in AB29.12 was set to 1. Error bars represent SE, n = 6, *P < 0.05, **P < 0.001. Blue arrows indicate the three P. deltoides PtGAUT12.1-OE lines (AB29.2, AB29.7, and AB29.12) selected for further analysesBack to article page