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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus

Fig. 7

Plant diameter, transcript, lignin, saccharification, and cell wall composition of field-grown P. deltoides control and PtGAUT12.1-OE plants. A Plant diameter and B debarked stem radial diameter of field-grown PtGAUT12.1-OE plants compared to controls. C Relative abundance of GAUT12.1 transcript determined by quantitative RT-PCR of RNA extracted from stems of 2.8-year-old field-grown trees and normalized to 18S. Expression of PdGAUT12.2 in AB29.7 was set to 1. Each data point represents mean ± SD of two biological replicates and three technical replicates, n = 4. D Total lignin content and E S/G ratio of WT, vector control, and PtGAUT12.1-OE lines. F Glucose, G xylose, and H total sugar release from WT and transgenic lines. I Galacturonic acid (GalA) and J xylose (Xyl) contents of AIR from stem of 2.8-year-old field-grown WT and PtGAUT12.1-OE plants. n = 4. Error bars represent SE. *P < 0.05, **P < 0.001

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