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Table 1 Glycosyl residue composition of alcohol insoluble residue (AIR) from stems of greenhouse-grown, 9-month-old P. deltoides WT, vector control, and PtGAUT12.1-OE lines of tetramethylsilane (TMS) derivatives

From: Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus

 

Glycosyl residue composition of AIR (mol% ± SE)

Ara

Rha

Fuc

Xyl

GlcA

GalA

Man

Gal

Glc

WT

3.06 ± 0.06

1.64 ± 0.06

0.09 ± 0.006

47.78 ± 1.21

0.21 ± 0.008

7.77 ± 0.21

6.08 ± 0.25

2.89 ± 0.14

30.48 ± 1.20

V Control-1

3.01 ± 0.08

1.79 ± 0.04

0.08 ± 0.004

47.26 ± 1.32

0.22 ± 0.006

6.97 ± 0.14

6.43 ± 0.28

3.01 ± 0.21

31.23 ± 1.32

AB29.2

2.96 ± 0.10

1.95 ± 0.06

0.07 ± 0.007

54.28 ± 1.01*

0.20 ± 0.010

8.67 ± 0.22*

4.11 ± 0.33*

2.11 ± 0.16*

25.65 ± 1.11*

AB29.7

2.88 ± 0.07

2.01 ± 0.05

0.10 ± 0.006

55.64 ± 1.11*

0.21 ± 0.005

8.96 ± 0.23*

4.23 ± 0.27*

1.97 ± 0.18*

24.00 ± 1.21*

AB29.12

2.57 ± 0.09

2.22 ± 0.08

0.08 ± 0.010

57.43 ± 0.98**

0.19 ± 0.007

9.11 ± 0.24**

3.15 ± 0.41**

1.05 ± 0.21**

24.20 ± 1.25*

  1. The amounts of sugar are represented as average mol% of AIR ± SE of 3 biological and 2 technical replicates (n = 6). Italics numbers with stars indicate mutant values that are significantly different from WT at * P ≤ 0.05, ** P ≤ 0.001 significant level (one-way ANOVA followed by Tukey’s multiple comparison test)