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Table 1 Glycosyl residue composition of alcohol insoluble residue (AIR) from stems of greenhouse-grown, 9-month-old P. deltoides WT, vector control, and PtGAUT12.1-OE lines of tetramethylsilane (TMS) derivatives

From: Working towards recalcitrance mechanisms: increased xylan and homogalacturonan production by overexpression of GAlactUronosylTransferase12 (GAUT12) causes increased recalcitrance and decreased growth in Populus

  Glycosyl residue composition of AIR (mol% ± SE)
Ara Rha Fuc Xyl GlcA GalA Man Gal Glc
WT 3.06 ± 0.06 1.64 ± 0.06 0.09 ± 0.006 47.78 ± 1.21 0.21 ± 0.008 7.77 ± 0.21 6.08 ± 0.25 2.89 ± 0.14 30.48 ± 1.20
V Control-1 3.01 ± 0.08 1.79 ± 0.04 0.08 ± 0.004 47.26 ± 1.32 0.22 ± 0.006 6.97 ± 0.14 6.43 ± 0.28 3.01 ± 0.21 31.23 ± 1.32
AB29.2 2.96 ± 0.10 1.95 ± 0.06 0.07 ± 0.007 54.28 ± 1.01* 0.20 ± 0.010 8.67 ± 0.22* 4.11 ± 0.33* 2.11 ± 0.16* 25.65 ± 1.11*
AB29.7 2.88 ± 0.07 2.01 ± 0.05 0.10 ± 0.006 55.64 ± 1.11* 0.21 ± 0.005 8.96 ± 0.23* 4.23 ± 0.27* 1.97 ± 0.18* 24.00 ± 1.21*
AB29.12 2.57 ± 0.09 2.22 ± 0.08 0.08 ± 0.010 57.43 ± 0.98** 0.19 ± 0.007 9.11 ± 0.24** 3.15 ± 0.41** 1.05 ± 0.21** 24.20 ± 1.25*
  1. The amounts of sugar are represented as average mol% of AIR ± SE of 3 biological and 2 technical replicates (n = 6). Italics numbers with stars indicate mutant values that are significantly different from WT at * P ≤ 0.05, ** P ≤ 0.001 significant level (one-way ANOVA followed by Tukey’s multiple comparison test)