Fig. 5From: Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetateEffects of different carbon sources on isobutanol production in engineered P. pastoris. Total isobutanol production in minimum yeast media with either 2% glycerol (a) or 2% glucose (b) as the sole carbon source. Specific isobutanol production in minimum yeast media with either 2% glycerol (c) or 2% glucose (d) as the sole carbon source. Engineered strains (PP100, PP200, PP300) were pre-cultured in 5-mL aliquots in MGYH (2% glycerol) minimal medium overnight and used to inoculate either 5 mL fresh MGYH (2% glycerol, a, c) or 5 mL fresh MGYH_glu (2% glucose, b, d) to achieve an initial optical density of 0.05 at 600 nm (OD600). The cultures were grown at 30 °C and 250 rpm in an orbital shaking incubator. Samples were taken at 24 and 48 h time points and the supernatants were analyzed on HPLC to quantify the isobutanol content. Values are the mean of three biological replicates ± standard deviation (n = 3)Back to article page