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Fig. 5 | Biotechnology for Biofuels

Fig. 5

From: Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate

Fig. 5

Effects of different carbon sources on isobutanol production in engineered P. pastoris. Total isobutanol production in minimum yeast media with either 2% glycerol (a) or 2% glucose (b) as the sole carbon source. Specific isobutanol production in minimum yeast media with either 2% glycerol (c) or 2% glucose (d) as the sole carbon source. Engineered strains (PP100, PP200, PP300) were pre-cultured in 5-mL aliquots in MGYH (2% glycerol) minimal medium overnight and used to inoculate either 5 mL fresh MGYH (2% glycerol, a, c) or 5 mL fresh MGYH_glu (2% glucose, b, d) to achieve an initial optical density of 0.05 at 600 nm (OD600). The cultures were grown at 30 °C and 250 rpm in an orbital shaking incubator. Samples were taken at 24 and 48 h time points and the supernatants were analyzed on HPLC to quantify the isobutanol content. Values are the mean of three biological replicates ± standard deviation (n = 3)

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