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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Metabolic engineering of Pichia pastoris for production of isobutanol and isobutyl acetate

Fig. 7

Effects of glucose concentration in the culture media on total isobutanol production in engineered P. pastoris. The engineered strain PP300 was pre-cultured in 5-mL aliquots in MGYH (2% glycerol) minimal medium overnight and used to inoculate either 50 mL fresh MGYH_glu (2% glucose) (a) or 50 mL fresh MGYH_glu (10% glucose) (b) in 250 mL Erlenmeyer flasks to achieve an initial optical density of 0.05 at 600 nm (OD600). The cultures were grown at 30 °C and 250 rpm in an orbital shaking incubator. Samples were taken at several time points and the supernatants were analyzed on HPLC to quantify the levels of isobutanol, glucose and other metabolites. Values are the mean of three biological replicates ± standard deviation (n = 3)

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