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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Correlation of structure, function and protein dynamics in GH7 cellobiohydrolases from Trichoderma atroviride, T. reesei and T. harzianum

Fig. 3

Real-time progress curves of cellulose hydrolysis. Cellobiose production during initial hydrolysis of BMCC by TreCel7A, TreCel7A_CD, TatCel7A, TatCel7A_CD, and ThaCel7A enzymes was monitored with an amperometric CDH enzyme biosensor. The reactions were carried out at 25 °C, pH 5.0, with 3.3 g/L of BMCC and 50 nM enzyme. Each enzyme kinetic curve was measured in duplicate, and the average curve is plotted for each enzyme. For more details, see Additional file 1: Figure S3

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