Fig. 1From: Gene stacking of multiple traits for high yield of fermentable sugars in plant biomassjStack constructs for the generation of multiple traits engineered Arabidopsis. Lines W0, W1, W2, W3, W4 and W5 express constructs C0, C1, C2, C3, C4 and C5, respectively, in Col-0 WT background. Lines X0 and X4 express constructs C0 and C4, respectively, in irx7/irx7 pVND7:IRX7 xylan-engineered (XE) background. White squares represent galactan biosynthesis-related ORF, gray square represents secondary cell wall artificial-positive feedback loop-related ORF, and black square represents decreasing lignin-related ORF. pNOS, pMAS and pACT2 are constitutive promoters, and pC4H, pIRX5, pIRX8 and pCESA7 are promoters specifically expressed in secondary cell wall producing cells. The foot-and-mouth disease virus 2A sequence ensures NST1 and UGE2 protein coordinated expressionBack to article page