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Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: Gene stacking of multiple traits for high yield of fermentable sugars in plant biomass

Fig. 1

jStack constructs for the generation of multiple traits engineered Arabidopsis. Lines W0, W1, W2, W3, W4 and W5 express constructs C0, C1, C2, C3, C4 and C5, respectively, in Col-0 WT background. Lines X0 and X4 express constructs C0 and C4, respectively, in irx7/irx7 pVND7:IRX7 xylan-engineered (XE) background. White squares represent galactan biosynthesis-related ORF, gray square represents secondary cell wall artificial-positive feedback loop-related ORF, and black square represents decreasing lignin-related ORF. pNOS, pMAS and pACT2 are constitutive promoters, and pC4H, pIRX5, pIRX8 and pCESA7 are promoters specifically expressed in secondary cell wall producing cells. The foot-and-mouth disease virus 2A sequence ensures NST1 and UGE2 protein coordinated expression

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