Fig. 1

Benchmarking of GFP expression in P. thermoglucosidasius DSM 2542. a Expression of different GFP variants from pNW-Ppta-GFPx-3TER plasmids at 53 °C in TGP broth was analyzed by flow cytometry at the mid-exponential phase of growth. The mean fluorescence intensity (MFI) of 50,000 cells is shown. Control, autofluorescence of DSM 2542 containing a pNW-Ppta-3TER plasmids without gfp gene (b). Temperature-dependent performance of sfGFP(Sp) as assessed flow cytometry. The MFI of 50,000 cells at the mid-exponential phase of growth cells grown at indicated temperatures in TGP broth is presented. The averages of three biological triplicates are shown with error bars indicating standard deviations