Skip to main content

Advertisement

Fig. 1 | Biotechnology for Biofuels

Fig. 1

From: In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria

Fig. 1

Benchmarking of GFP expression in P. thermoglucosidasius DSM 2542. a Expression of different GFP variants from pNW-Ppta-GFPx-3TER plasmids at 53 °C in TGP broth was analyzed by flow cytometry at the mid-exponential phase of growth. The mean fluorescence intensity (MFI) of 50,000 cells is shown. Control, autofluorescence of DSM 2542 containing a pNW-Ppta-3TER plasmids without gfp gene (b). Temperature-dependent performance of sfGFP(Sp) as assessed flow cytometry. The MFI of 50,000 cells at the mid-exponential phase of growth cells grown at indicated temperatures in TGP broth is presented. The averages of three biological triplicates are shown with error bars indicating standard deviations

Back to article page