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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: In vivo selection of sfGFP variants with improved and reliable functionality in industrially important thermophilic bacteria

Fig. 2

Topology map of sfGFP with thermostability-enhancing mutations isolated from in vivo FACS screening of DSM 2542. Left side: mutations found in the 50 sequenced mutants obtained by cell sorting from the sfGFP(Sp) library. Green stars: mutations found in at least 1 out of 50 protein sequences; blue stars: mutations that map to loop sites or flexible linker regions; orange stars: mutations that occurred in more than 10 out of 50 sfGFP(Sp) variants. Yellow cloud: chromophore

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