Fig. 5

Visualization of sfGFP(N39D/A179A) expression in different thermophilic spore-forming species by fluorescence microscopy. Expression of the thermostable GFP variant sfGFP(N39D/A179A) from the plasmid pNW-Ppta-sfGFP(N39D/A179A)-3TER was imaged after growing the transformed strains on growth media as specified in “Methods”. a P. thermoglucosidasius DSM 2542 (60 °C), b B. smithii DSM 4216 (55 °C), c B. coagulans DSM 1 (50 °C), d B. methanolicus DSM 16454 (60 °C), e G. thermodenitrificans DSM 465 (60 °C), f G. thermodenitrificans T12 (60 °C). Excitation time, excitation and emission wavelengths and filter settings are given in “Methods”