Fig. 6

Visualization of expression of FP color variants in different (moderate) thermophilic spore-forming species. Expression of the cyan variant sfCFP(N39D/A179A) and the yellow variant sfYFP(N39D/A179A) from the corresponding pNW-P_pta-GFPx-3TER plasmids was monitored after growing the transformed strains at their indicated optimal growth temperatures. a, e P. thermoglucosidasius DSM 2542 (60 °C), b, f B. smithii DSM 4216 (55 °C), c, g B. coagulans DSM 1 (50 °C), d, h G. thermodenitrificans T12 (60 °C). For excitation time, excitation and emission wavelengths and filter settings see “Methods”