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Table 1 Bacterial strains and plasmids used in this study

From: Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum

Strains/plasmids Relevant characteristic Sources
 E. coli
  DH5α f80dlacZΔM15, Δ(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rk − , mk +), phoA, supE44, l − , thi-1, gyrA96, relA1 Transgen
  BL21(DE3) ompT gal dcm lon hsdSB(rB − mB −) l (DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) Transgen
  BL21(DE3)::pET28aNS-rCel48S_CD Derived from BL21(DE3), carrying plasmid pET28aNS-rCel48S_CD to express the recombinant Cel48S_CD (rCel48S_CD) This study
 C. thermocellum
  ATCC27405 Wild type stain ATCC
  DSM1313 Wild type stain DSMZ
  ΔpyrF Derived from DSM1313, with deleted pyrF gene [32]
  ΔpyrF::Cel48S_CD-His12 Derived from ΔpyrF, expressing the native Cel48S_CD bearing a His12 tag (ctCel48S_CD -His12) This study
 pET28aNS Expression vector with N-terminal hexahistidine affinity tag, with modified multiple cloning sites [31]
 pET28aNS-rCel48S_CD pET28aNS derivative for expression of Cel48S_CD from C. thermocellum ATCC27405 This study
 pHK-HR-CaBglA Markerless genome editing plasmid for DSM 1313 [32]
 pHK-HR-His12TAA pHK-HR-CaBglA derivative for markerless insertion of His12TAA in the chromosome of DSM 1313 This study