Fig. 12

Enhanced cellulase secretion and activity was detected in PfMig1⁸⁸ in production medium. a Growth of the fungus in production media was monitored for 7 days by estimating the mycelial proteins by BCA method. RCM in X-axis denotes the amount of protein present in the pellet of medium before inoculation. b Proteins present in the secretome of NCIM1228 (upper panel) and PfMig1⁸⁸ (lower panel) were collected for each day and separated on 10% SDS-PAGE gel followed by staining with Coomassie blue. c Secretome proteins of NCIM1228 and PfMig1⁸⁸ collected for each day was measured by BCA method after exchanging growth medium with citrate–phosphate buffer (pH 4.0). RCM in X-axis denotes the amount of protein present in the supernatant of medium before inoculation. d Total cellulase activity profile represented by FPU/ml of NCIM1228 and PfMig1⁸⁸ during 7 days of cultivation