Fig. 2

PfMig1 has a stop codon at 134th amino acid position. a Diagrammatic representation of putative domains detected in PfMig1. Red line states the presence of nonsense mutation at 134th amino acid position just before the alanine-rich region, thus naming the allele as Mig1¹³⁴. b Sequence alignment of Mig1 protein of closely related fungi with PfMig1. The alignment indicates that the stop codon at 134th position was not due to natural selection, but was rather due to the result of laboratory induced mutation. Sequence from amino acid position 79–139 was taken as query. Red box indicates the 134th position corresponding to stop codon of PfMig1