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Fig. 7 | Biotechnology for Biofuels

Fig. 7

From: Disruption of zinc finger DNA binding domain in catabolite repressor Mig1 increases growth rate, hyphal branching, and cellulase expression in hypercellulolytic fungus Penicillium funiculosum NCIM1228

Fig. 7

Presence of PfMig188 allele induces profuse hyphal branching. a Colony morphology of NCIM1228 and PfMig188 after 10 days of incubation. Black arrow denotes the radius of the whole colony, blue arrow denotes the extent of core and middle layer, and red arrow shows highly extended invading layer in NCIM1228 and highly reduced outer layer in PfMig188. The outer layer of NCIM1228 and PfMig188 cells were observed under fluorescence microscope at ×4 magnification (b) and at ×20 magnification (c). In NCIM1228, parallel grouped hyphal structures are shown running throughout the outer layer, whereas no such structures were observed in PfMig188. d Graphical representation of colony morphology observed in NCIM1228 and PfMig188. NCIM1228 colonies are organized around parallel hyphae running from core of the colony toward periphery, whereas no such impeded organization was observed in PfMig188. e Branching pattern observed in terminal hyphae of NCIM1228 and PfMig188 colonies

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