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Fig. 9 | Biotechnology for Biofuels

Fig. 9

From: Disruption of zinc finger DNA binding domain in catabolite repressor Mig1 increases growth rate, hyphal branching, and cellulase expression in hypercellulolytic fungus Penicillium funiculosum NCIM1228

Fig. 9

Secretome profiling of NCIM1228 and PfMig188 in the presence of glucose. a Upper panel shows SDS-PAGE profile of 10 times diluted secretome of NCIM1228 and PfMig188 grown in Mandel media having varying ratios of glucose and Avicel. Lower panel shows detection of CBHI under different conditions by Western blotting using rabbit anti-CBHI primary antibody and HRPO-conjugated anti-rabbit secondary antibody. Activities for b Avicelase, c CMCase, d xylanase, e β-glucosidase, and f FPase were measured in the secretome of NCIM1228 and PfMig188 when grown under different ratios of glucose and Avicel for 5 days

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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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