Fig. 1

The enzyme hydrolytic performance of different cellulose substrates. a Cellobiose accumulation. b cellobiose/glucose ratio from cellulose by TrCel7A alone (12 mg enzyme/g glucan). c TOF of cellulase at different enzyme loadings. aTOF is apparent turnover frequency, which is defined as the mole number of glucose production per hour per gram enzyme at initial hydrolytic stage. d Effect of cellulases loadings on glucose release for 72-h hydrolysis. e The kinetics curve of glucose release during enzymatic hydrolysis under the enzyme loading of 20 mg protein/g-cellulose. Other conditions are as follows: cellulose substrate concentration 1%, 50 mM acetate acid buffer (pH 5.0), cellulases complexes of 90% Celluclast 1.5 L and 10% β-glucosidase