Table 1 Results of the systematic optimization of styrene biosynthesis in E. coli BL21(DE3)

1

Enzyme screening and codon optimization

E. coli BL01

pACYC-AtPAL-FDC1

–

–

2

Plasmid optimization

E. coli BL05

pTrc-AtPAL-FDC1

3

Co-expression of aroF and pheA

E. coli BL0501

pTrc-AtPAL-FDC1 and pACYC-aroF-pheA

4

Co-expression of aroF, pheA, ppsA, and tktA

E. coli BL0801

pTrc-AtPAL-FDC1-ppsA-tktA and pACYC-aroF-pheA

5

Expression time optimization

E. coli BL0801

pTrc-AtPAL-FDC1-ppsA-tktA and pACYC-aroF-pheA

6

In situ extraction

E. coli BL0801

pTrc-AtPAL-FDC1-ppsA-tktA and pACYC-aroF-pheA-tktA-ppsA