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Table 1 Results of the systematic optimization of styrene biosynthesis in E. coli BL21(DE3)

From: A systematic optimization of styrene biosynthesis in Escherichia coli BL21(DE3)

Step Optimization strategy Strain Plasmid Yield
(mg/L)
Improvement
over previous step (%)
Improvement
over original step (%)
1 Enzyme screening and codon optimization E. coli BL01 pACYC-AtPAL-FDC1 55
2 Plasmid optimization E. coli BL05 pTrc-AtPAL-FDC1 103 187 187
3 Co-expression of aroF and pheA E. coli BL0501 pTrc-AtPAL-FDC1 and pACYC-aroF-pheA 210 203 382
4 Co-expression of aroF, pheA, ppsA, and tktA E. coli BL0801 pTrc-AtPAL-FDC1-ppsA-tktA and pACYC-aroF-pheA 275 131 500
5 Expression time optimization E. coli BL0801 pTrc-AtPAL-FDC1-ppsA-tktA and pACYC-aroF-pheA 275 100 500
6 In situ extraction E. coli BL0801 pTrc-AtPAL-FDC1-ppsA-tktA and pACYC-aroF-pheA-tktA-ppsA 350 127 636