From: A systematic optimization of styrene biosynthesis in Escherichia coli BL21(DE3)
Name | Relevant characteristics | References |
---|---|---|
Plasmids | ||
 pACYCDuet-1 | P15A origin; CmR; PT7 | Novagen |
 pACYCDuet-AtPAL-FDC1 | P15A origin; CmR; PT7-AtPAL-FDC1 | This work |
 pACYCDuet-AaPAL-FDC1 | P15A origin; CmR; PT7-AaPAL-FDC1 | This work |
 pACYCDuet-FtPAL-FDC1 | P15A origin; CmR; PT7-FtPAL-FDC1 | This work |
 pACYCDuet-PcPAL-FDC1 | P15A origin; CmR; PT7-PcPAL-FDC1 | This work |
 pACYCDuet-aroF-pheA | P15A origin; CmR; PT7-aroF-pheA | This work |
 pTrcHis2B | pBR322 origin; AmpR; PTrc | Novagen |
 pTrcHis2B-AtPAL-FDC1 | pBR322 origin; AmpR; PTrc-AtPAL-FDC1 | This work |
 pTrcHis2B-AtPAL-FDC1-ppsA-tktA | pBR322 origin; AmpR; PTrc-AtPAL-FDC1-ppsA-tktA | This work |
 pET-28a | f1 origin; KanR; PT7 | Novagen |
 pET-28a-AtPAL-FDC1 | f1 origin; KanR; PT7-AtPAL-FDC1 | This work |
 pColiDuet-1 | ColA origin; KanR; PT7-AtPAL-FDC1 | Novagen |
 pColiDuet-AtPAL-FDC1 | ColA origin; KanR; PT7 | This work |
Strains | ||
 E. coli BL21(DE3) | E. coli B dcm ompT hsdS(rB− mB−) gal | Takara |
 E. coli DH5α | deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ−, thi−1, gyrA96, relA1 | Invitrogen |
 BL01 | E. coli BL21(DE3)-harboring pACYCDuet-AtPAL-FDC1 | This work |
 BL02 | E. coli BL21(DE3)-harboring pACYCDuet-AaPAL-FDC1 | This work |
 BL03 | E. coli BL21(DE3)-harboring pACYCDuet-FtPAL-FDC1 | This work |
 BL04 | E. coli BL21(DE3)-harboring pACYCDuet-PcPAL-FDC1 | This work |
 BL05 | E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1 | This work |
 BL06 | E. coli BL21(DE3)-harboring pET-28a-AtPAL-FDC1 | This work |
 BL07 | E. coli BL21(DE3)-harboring pColADuet-AtPAL-FDC1 | This work |
 BL0500 | E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1 and pACYCDuet-1 | This work |
 BL0501 | E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1 and pACYCDuet-aroF-pheA | This work |
 BL08 | E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1 -ppsA-tktA | This work |
 BL0801 | E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1-ppsA-tktA and pACYCDuet-aroF-pheA | This work |