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Table 2 Plasmids and strains used in this study

From: A systematic optimization of styrene biosynthesis in Escherichia coli BL21(DE3)

Name Relevant characteristics References
Plasmids
 pACYCDuet-1 P15A origin; CmR; PT7 Novagen
 pACYCDuet-AtPAL-FDC1 P15A origin; CmR; PT7-AtPAL-FDC1 This work
 pACYCDuet-AaPAL-FDC1 P15A origin; CmR; PT7-AaPAL-FDC1 This work
 pACYCDuet-FtPAL-FDC1 P15A origin; CmR; PT7-FtPAL-FDC1 This work
 pACYCDuet-PcPAL-FDC1 P15A origin; CmR; PT7-PcPAL-FDC1 This work
 pACYCDuet-aroF-pheA P15A origin; CmR; PT7-aroF-pheA This work
 pTrcHis2B pBR322 origin; AmpR; PTrc Novagen
 pTrcHis2B-AtPAL-FDC1 pBR322 origin; AmpR; PTrc-AtPAL-FDC1 This work
 pTrcHis2B-AtPAL-FDC1-ppsA-tktA pBR322 origin; AmpR; PTrc-AtPAL-FDC1-ppsA-tktA This work
 pET-28a f1 origin; KanR; PT7 Novagen
 pET-28a-AtPAL-FDC1 f1 origin; KanR; PT7-AtPAL-FDC1 This work
 pColiDuet-1 ColA origin; KanR; PT7-AtPAL-FDC1 Novagen
 pColiDuet-AtPAL-FDC1 ColA origin; KanR; PT7 This work
Strains
 E. coli BL21(DE3) E. coli B dcm ompT hsdS(rB mB) gal Takara
 E. coli DH5α deoR, recA1, endA1, hsdR17(rk, mk+), phoA, supE44, λ, thi−1, gyrA96, relA1 Invitrogen
 BL01 E. coli BL21(DE3)-harboring pACYCDuet-AtPAL-FDC1 This work
 BL02 E. coli BL21(DE3)-harboring pACYCDuet-AaPAL-FDC1 This work
 BL03 E. coli BL21(DE3)-harboring pACYCDuet-FtPAL-FDC1 This work
 BL04 E. coli BL21(DE3)-harboring pACYCDuet-PcPAL-FDC1 This work
 BL05 E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1 This work
 BL06 E. coli BL21(DE3)-harboring pET-28a-AtPAL-FDC1 This work
 BL07 E. coli BL21(DE3)-harboring pColADuet-AtPAL-FDC1 This work
 BL0500 E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1 and pACYCDuet-1 This work
 BL0501 E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1 and pACYCDuet-aroF-pheA This work
 BL08 E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1 -ppsA-tktA This work
 BL0801 E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1-ppsA-tktA and pACYCDuet-aroF-pheA This work