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Table 2 Plasmids and strains used in this study

From: A systematic optimization of styrene biosynthesis in Escherichia coli BL21(DE3)

Name

Relevant characteristics

References

Plasmids

 pACYCDuet-1

P15A origin; CmR; PT7

Novagen

 pACYCDuet-AtPAL-FDC1

P15A origin; CmR; PT7-AtPAL-FDC1

This work

 pACYCDuet-AaPAL-FDC1

P15A origin; CmR; PT7-AaPAL-FDC1

This work

 pACYCDuet-FtPAL-FDC1

P15A origin; CmR; PT7-FtPAL-FDC1

This work

 pACYCDuet-PcPAL-FDC1

P15A origin; CmR; PT7-PcPAL-FDC1

This work

 pACYCDuet-aroF-pheA

P15A origin; CmR; PT7-aroF-pheA

This work

 pTrcHis2B

pBR322 origin; AmpR; PTrc

Novagen

 pTrcHis2B-AtPAL-FDC1

pBR322 origin; AmpR; PTrc-AtPAL-FDC1

This work

 pTrcHis2B-AtPAL-FDC1-ppsA-tktA

pBR322 origin; AmpR; PTrc-AtPAL-FDC1-ppsA-tktA

This work

 pET-28a

f1 origin; KanR; PT7

Novagen

 pET-28a-AtPAL-FDC1

f1 origin; KanR; PT7-AtPAL-FDC1

This work

 pColiDuet-1

ColA origin; KanR; PT7-AtPAL-FDC1

Novagen

 pColiDuet-AtPAL-FDC1

ColA origin; KanR; PT7

This work

Strains

 E. coli BL21(DE3)

E. coli B dcm ompT hsdS(rB− mB−) gal

Takara

 E. coli DH5α

deoR, recA1, endA1, hsdR17(rk−, mk+), phoA, supE44, λ−, thi−1, gyrA96, relA1

Invitrogen

 BL01

E. coli BL21(DE3)-harboring pACYCDuet-AtPAL-FDC1

This work

 BL02

E. coli BL21(DE3)-harboring pACYCDuet-AaPAL-FDC1

This work

 BL03

E. coli BL21(DE3)-harboring pACYCDuet-FtPAL-FDC1

This work

 BL04

E. coli BL21(DE3)-harboring pACYCDuet-PcPAL-FDC1

This work

 BL05

E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1

This work

 BL06

E. coli BL21(DE3)-harboring pET-28a-AtPAL-FDC1

This work

 BL07

E. coli BL21(DE3)-harboring pColADuet-AtPAL-FDC1

This work

 BL0500

E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1 and pACYCDuet-1

This work

 BL0501

E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1 and pACYCDuet-aroF-pheA

This work

 BL08

E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1 -ppsA-tktA

This work

 BL0801

E. coli BL21(DE3)-harboring pTrcHis2B-AtPAL-FDC1-ppsA-tktA and pACYCDuet-aroF-pheA

This work