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Table 3 Primers used in this study

From: A systematic optimization of styrene biosynthesis in Escherichia coli BL21(DE3)

Primers Nucleotide sequencea Description
aroF-F GCggatccTATGCAAAAAGACGCGCTGAAT Start of aroF; forward primer
aroF-R ATAAGAATgcggccgcTTAAGCCACGCGAGCCGT End of aroF; reverse primer
pheA-F GAagatctcATGACATCGGAAAACCCGTTAC Start of pheA; forward primer
pheA-R CCGctcgagTTAGGTTGGATCAACAGGCAC End of pheA; reverse primer
ppsA-F CGATCGCTGACGTCGGTACCctcgagTTAAGGAGGTATATATTAATGTCCAACAATGGCTCGT Start of ppsA; forward primer
ppsA-R CTTTACGTGAGGACATTAATATATACCTCCTTAAaagcttTTATTTCTTCAGTTCAGCCAGG End of ppsA; reverse primer
tktA-F TGGCTGAACTGAAGAAATAAaagcttTTAAGGAGGTATATATTAATGTCCTCACGTAAAGAGCTT Start of tktA; forward primer
tktA-R CTTCTGAGATGAGTTTTTGTtctagaTTACAGCAGTTCTTTTGCTTT End of tktA; reverse primer
  1. aRestriction sites are marked in lower case letters. RBS are underline