Table 3 Primers used in this study
From: A systematic optimization of styrene biosynthesis in Escherichia coli BL21(DE3)
Primers | Nucleotide sequencea | Description |
|---|---|---|
aroF-F | GCggatccTATGCAAAAAGACGCGCTGAAT | Start of aroF; forward primer |
aroF-R | ATAAGAATgcggccgcTTAAGCCACGCGAGCCGT | End of aroF; reverse primer |
pheA-F | GAagatctcATGACATCGGAAAACCCGTTAC | Start of pheA; forward primer |
pheA-R | CCGctcgagTTAGGTTGGATCAACAGGCAC | End of pheA; reverse primer |
ppsA-F | CGATCGCTGACGTCGGTACCctcgagTTAAGGAGGTATATATTAATGTCCAACAATGGCTCGT | Start of ppsA; forward primer |
ppsA-R | CTTTACGTGAGGACATTAATATATACCTCCTTAAaagcttTTATTTCTTCAGTTCAGCCAGG | End of ppsA; reverse primer |
tktA-F | TGGCTGAACTGAAGAAATAAaagcttTTAAGGAGGTATATATTAATGTCCTCACGTAAAGAGCTT | Start of tktA; forward primer |
tktA-R | CTTCTGAGATGAGTTTTTGTtctagaTTACAGCAGTTCTTTTGCTTT | End of tktA; reverse primer |