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Table 3 Scale-up lipid fermentation strategies of lignin stream by engineered Rhodococcus opacus PD630_FA in a 2.0-l fermenter

From: Combinatorial pretreatment and fermentation optimization enabled a record yield on lignin bioconversion

Experiment no. Substrate Laccase treated Fermentation mode Initial OD SSC (g/l) Total fermentation time (h) Nitrogen source (g/l)
1 Lignin 1 No Batch 1.0 10 at 0 h 96 1.4
2 Lignin 1 No Batch 10 40 at 0 h 96 1.4
3 Lignin 1 No Fed-batch 10 20 at 0 h + 20 at 72 h 168 1.4
4 Lignin 4 No Batch 1.0 10 at 0 h 96 1.4
5 Lignin 4 No Fed-batch 5.0 20 at 0 h + 20 at 72 h 168 1.4
6 Lignin 4 Yes Fed-batch 5.0 20 at 0 h + 20 at 72 h 168 1.4
  1. Lignins 1 and 4 represent the lignin substrate fractionated by pretreatment Cases 1 and 4, as described in Table 1, respectively
  2. SSC soluble substrate concentration