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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Processivity and enzymatic mechanism of a multifunctional family 5 endoglucanase from Bacillus subtilis BS-5 with potential applications in the saccharification of cellulosic substrates

Fig. 2

Effect of temperature and pH on the activity and stability of EG5C and its derivatives. a Activity of EG5C (), EG5C-1 (□) and EG5C-2 (∆) was measured at different pH values at 45 °C. b pH stability of EG5C (), EG5C-1 (□) and EG5C-2 (∆) evaluated following incubation at 45 °C for 2 h at different pH values. The buffer systems were as follows: 50 mmol/L citric acid/sodium citrate (pH 3.0–6.0), 50 mmol/L Na2HPO4/KH2PO4 (pH 6.0–8.5), 50 mmol/L Gly/NaOH (pH 8.5–10.5). c The effect of temperature on activity of EG5C (), EG5C-1 (□) and EG5C-2 (∆) was studied by changing the temperature from 30 to 80 °C at pH 6.5. d Thermosatbility of EG5C (), EG5C-1 (□) and EG5C-2 (∆) measured by incubating the enzyme at various temperatures for 2 h. Error bars are standard deviation of 3 independent experiments

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