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Table 2 Kinetic parameters of EG5C and its derivatives at optimal reaction condition

From: Processivity and enzymatic mechanism of a multifunctional family 5 endoglucanase from Bacillus subtilis BS-5 with potential applications in the saccharification of cellulosic substrates

Substrate EG5C EG5C-1 EG5C-2
Km (mg/mL) kcat (/s) kcat/Km (mL/mg/s) Km (mg/mL) kcat (/s) kcat/Km (mL/mg/s) Km (mg/mL) kcat (/s) kcat/Km (mL/mg/s)
CMC 5.7 218 38 6.5 193 30 5.9 145 25
PASC N N N 22.7 41.3 1.6 N N N
Avicel N N N 74.6 2.4 0.1 N N N
  1. N means not detected. Kinetic study was performed at 60 °C and pH 6.0. The data were analyzed using non-linear regression with GraphPad Prism 5.0 software