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Table 1 Biological methane potential (BMP) test experimental setup

From: Enhancing methane production from lignocellulosic biomass by combined steam-explosion pretreatment and bioaugmentation with cellulolytic bacterium Caldicellulosiruptor bescii

Name Substrate Inoculum C. bescii culture (v/v), %c
Untreated + 0% Untreated birch +a b
Pretreated + 0% Pretreated birch +
Pretreated + 2% Pretreated birch + 2
Pretreated + 5% Pretreated birch + 5
Pretreated + 10% Pretreated birch + 10
Pretreated + 15% Pretreated birch + 15
Inoculum + 0% +
Inoculum + 2% + 2
Inoculum + 5% + 5
Inoculum + 10% + 10
Inoculum + 15% + 15
  1. aThe positive sign represents that all the bottles received the same amount of inoculum
  2. bThe negative sign represents that these bottles did not receive the indicated materials (C. bescii culture and/or substrate)
  3. cThe volume of C. bescii culture divided by the total volume of the solution (70-mL working volume). Optical density (680 nm) of the culture was 0.54