Enhancing methane production from lignocellulosic biomass by combined steam-explosion pretreatment and bioaugmentation with cellulolytic bacterium Caldicellulosiruptor bescii

Table 1 Biological methane potential (BMP) test experimental setup

Name	Substrate	Inoculum	C. bescii culture (v/v), %^c
Untreated + 0%	Untreated birch	+^a	–^b
Pretreated + 0%	Pretreated birch	+	–
Pretreated + 2%	Pretreated birch	+	2
Pretreated + 5%	Pretreated birch	+	5
Pretreated + 10%	Pretreated birch	+	10
Pretreated + 15%	Pretreated birch	+	15
Inoculum + 0%	–	+	–
Inoculum + 2%	–	+	2
Inoculum + 5%	–	+	5
Inoculum + 10%	–	+	10
Inoculum + 15%	–	+	15

^aThe positive sign represents that all the bottles received the same amount of inoculum
^bThe negative sign represents that these bottles did not receive the indicated materials (C. bescii culture and/or substrate)
^cThe volume of C. bescii culture divided by the total volume of the solution (70-mL working volume). Optical density (680 nm) of the culture was 0.54

ISSN: 2731-3654