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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Characterization and engineering of a dual-function diacylglycerol acyltransferase in the oleaginous marine diatom Phaeodactylum tricornutum

Fig. 2

Dynamics of DGATs and lipidomics of P. tricornutum following nitrogen depletion. a Absolute abundance of DGATs in wild-type P. tricornutum at late logarithmic phase. Y axis means the transcript number. The concentrations of pMD19T-PtDGAT1, pMD19T-PtDGAT2A, and pMD19T-PtWS/DGAT were determined and diluted to 10−1, 10−2, 10−3, 10−4, and 10−5 as templates in real-time PCR for standard curve. R2 with values above 0.99 and amplification efficiencies with values above 90% were obtained for PtDGAT1, DGAT2A, and WS/DGAT genes (amplification curves and melting curves are shown in Additional file 2: Figure S4). b Transcriptional dynamics of DGATs upon nitrogen depletion. To impose N deprivation, P. tricornutum cells in the stationary phase were collected, washed, and re-suspended with N-free f/2 medium at room temperature. Samples were collected at indicated periods for transcript analysis. c Dynamics of total lipids and TAG in P. tricornutum after nitrogen depletion. d Profiles of fatty acids in TAG in P. tricornutum after nitrogen depletion

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