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Fig. 2 | Biotechnology for Biofuels

Fig. 2

From: Improved photobio-H2 production regulated by artificial miRNA targeting psbA in green microalga Chlamydomonas reinhardtii

Fig. 2

Construction of amiRNA-targeting psbA gene. a Selection criteria of WMD3 designing amiRNA. b Position of amiRNA-D1 target region in psbA gene. Solid green boxes represent psbA exons. Open boxes represent psbA introns. Short lines under the gene sketch show the designed amiRNAs, and the green line is the chosen amiRNA-D1. Target sequence of psbA is shown in black, and the mature miRNA sequence is shown in green. c Relative H2-producing level detected by gas chromatography (GC) indicates the first screen of Chlamydomonas transformants containing two best scored amiRNA candidates. d Loop–stem structure of miRNA1162 precursor and amiRNA-D1. The mature parts (miRNA/miRNA* duplex) are shown in the box. The mature miRNA sequence is shown in green and the miRNA* sequence is shown in red

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