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Fig. 3 | Biotechnology for Biofuels

Fig. 3

From: Improved photobio-H2 production regulated by artificial miRNA targeting psbA in green microalga Chlamydomonas reinhardtii

Fig. 3

Verification of transformant using PCR and RT-PCR. a The PCR amplification results of amiRNA-D1 fragment in transgenic algal strain and in WT. b RT-PCR amplification results of amiRNA in transgenic algal strain and in WT. Lanes 1, 2: transgenic algal strain; lane 3: WT CC-849; M molecular marker. All the positive bands were purified and verified by sequencing. Full-length gels are presented in Additional file 1: Figure S1

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