Table 2 Primers used for amiRNA-D1 and psbA gene expression detections by qRT-PCR
Name | Primer | Sequence (5′ → 3′) |
|---|---|---|
psbA quantification | ||
D1-F | Forward primer | CGGTAATCGGTATTTGGT |
D2-R | Reverse primer | GCACGGTTGATGATGTCT |
Actin-F | Forward primer | ACCCCGTGCTGCTGACTG |
Actin-R | Reverse primer | ACGTTGAAGGTCTCGAACA |
amiRNA-D1 quantification | ||
miRNA-D1-F | Forward primer | TGTCGGTATGTTGCAGTAAGA |
RT-amiRNA-D1 | Primer for reverse-transcription | GTGCAGGGTCCGAGGTCAGAGCCACCTGGGCAATTTTTTTTTTTCTGTCT |
U4-F | Forward primer | CGGCGCAAAAGGCCCGACAGAAAT |
RT-U4 | Primer for reverse-transcription | GTGCAGGGTCCGAGGTCAGAGCCACCTGGGCAATTTTTTTTTTTATTTCTC |
Universal R | Universal reverse primer | CAGTGCAGGGTCCGAGGT |