Fig. 4

Screen of recombinant strains in shake flasks. a Time course of X33-SD in 500-mL shake flask containing 100 mL YP media with 40 g/L glucose. b Time course of X33-SDB in 500-mL shake flask containing 100 mL YP media with 40 g/L glucose. c Time course of X33-SDU in 500-mL shake flask containing 100 mL YP media with 40 g/L glucose. Cultivation last for 36 h. Error bars indicate the standard deviation of three replicate experiments