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Table 3 Comparison of metabolite production of three P. pastoris strains

From: Production of (2R, 3R)-2,3-butanediol using engineered Pichia pastoris: strain construction, characterization and fermentation

Strains X33-SD X33-SDB X33-SDU
OD600 27.15 ± 1α 29.68 ± 2.08α 27.23 ± 0.62α
2,3-BD (g/L) 12.2 ± 0.84α 8.04 ± 0.95β 7.44 ± 0.19β
Acetoin (g/L) 1.84 ± 0.76α 1.03 ± 0.09α 0.54 ± 0.07β
Glycerol (g/L) 6.42 ± 0.62α 3.14 ± 0.28β 2.94 ± 0.23β
Ethanol (g/L) 2.28 ± 0.36α 6.43 ± 0.08β 4.96 ± 0.07γ
  1. Cultivation of three strains was carried out in 500-mL shake flasks containing 100 mL YP media with 40 g/L glucose. Cultivation last for 36 h. Data indicates average values of triplicate experiments with standard deviation. Values with different Greek symbols between columns were significantly different (P < 0.05). ND indicates not detected