Production of (2R, 3R)-2,3-butanediol using engineered Pichia pastoris: strain construction, characterization and fermentation

Table 3 Comparison of metabolite production of three P. pastoris strains

Strains	X33-SD	X33-SDB	X33-SDU
OD₆₀₀	27.15 ± 1^α	29.68 ± 2.08^α	27.23 ± 0.62^α
2,3-BD (g/L)	12.2 ± 0.84^α	8.04 ± 0.95^β	7.44 ± 0.19^β
Acetoin (g/L)	1.84 ± 0.76^α	1.03 ± 0.09^α	0.54 ± 0.07^β
Glycerol (g/L)	6.42 ± 0.62^α	3.14 ± 0.28^β	2.94 ± 0.23^β
Ethanol (g/L)	2.28 ± 0.36^α	6.43 ± 0.08^β	4.96 ± 0.07^γ

Cultivation of three strains was carried out in 500-mL shake flasks containing 100 mL YP media with 40 g/L glucose. Cultivation last for 36 h. Data indicates average values of triplicate experiments with standard deviation. Values with different Greek symbols between columns were significantly different (P < 0.05). ND indicates not detected

ISSN: 2731-3654