Fig. 8

Results of the β-galactosidase activity measurement, growth patterns, malonyl-CoA quantitation, RT-PCR. a β-Galactosidase activities in WT-LacZS and WT-HMLacZS without (shown as “theo−”) or with (shown as “theo+”) 2 mM theophylline induction. The error bar represents the standard deviation of the three biological replicates for each sample. b Growth patterns for WT and WT-HMMA1(the*). Both WT and WT-HMMA1(the*) were induced with 2 mM theophylline at 48 h. The error bar represents the standard deviation of the three biological replicates for each sample. c Malonyl-CoA contents of WT and WT-HMMA1(the*) at 72 h. The error bar represents the standard deviation of the three biological replicates for each sample. d RT-PCR for WT-HMMA1(the*) with or without induction. Lane 1–6: 1 and 3 were the expression level of Asslr1511-micC and Asslr2023-micC with 2 mM theophylline induction, respectively; 2 and 4 were the expression level of Asslr1511-micC and Asslr2023-micC without induction, respectively; 4 and 6 were the expression level of 16S rRNA in WT-HMMA1(the*) with or without induction, respectively