Alignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth

Table 1 Plasmids and strains used in this study

Plasmids and strains	Relevant characteristics	References
pFL-SN	BioBrick “T” vector with SpeI and NheI on each side	[40]
pWD42	Amp^rKm^r, containing the selection cassette	[58]
pWD001	pFL-SN derivate, Amp^r, containing acs gene upstream and downstream homologous regions	This study
pWD002	pFL-SN derivate, Amp^rKm^r, containing acs gene upstream homologous region, selection cassette and downstream homologous region	This study
pWD003	pFL-SN derivate, Amp^r, containing ackA gene upstream and downstream homologous regions	This study
pWD004	pFL-SN derivate, Amp^rKm^r, containing ackA gene upstream homologous region, selection cassette and downstream homologous region	This study
Synechocystis sp. PCC6803	Synechocystis sp. PCC6803 wild type	D. Bhaya
WD023	Synechocystis sp. PCC6803 acs gene knock out mutant	This study
WD025	Synechocystis sp. PCC6803 ackA gene knock out mutant	This study
WD027	Synechocystis sp. PCC6803 acs and ackA double gene knock out mutant	This study

ISSN: 2731-3654