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Table 3 Predicted and experimentally observed growth rate and product yield of acetate on biomass in wild-type Synechocystis and derivative strains impaired in acetate metabolism

From: Alignment of microbial fitness with engineered product formation: obligatory coupling between acetate production and photoautotrophic growth

Strains

Growth rate (µ, h−1)

Acetate yield (Yp/x, mM gDW−1)

Model prediction

Measured

Model prediction

Measureda

Wild type

0.052

0.048 ± 0.002

0

0

Δacs

0.052

0.049 ± 0.001

0

0.201 ± 0.011

ΔackA

0.052

0.048 ± 0.001

0

0

Δacs ackA

0.052

0.047 ± 0.002

0.192

0.193 ± 0.007

  1. aMeasured yield was calculated based on data collected during the exponential cell growth phase (65–120 h) presented in Fig. 4b. Dry weight concentration was extrapolated from the OD730 measurements using a conversion coefficient determined in this same setup of 148 mg L−1 OD −1730 [41]