Fig. 2From: A copper-controlled RNA interference system for reversible silencing of target genes in Trichoderma reeseiKnockdown of pyr4 using P tcu1 -driven RNAi system was responsive to copper in agar plate. Equal amount of conidia spores from QM9414 and the P tcu1 –pyr4KD strain was inoculated on minimal media agar plate containing 2% (w/v) glucose as a sole carbon source. a No copper and uridine was present in the media. Twenty μM copper (b) and 10 mM uridine (c) were added into the minimal media agar plates, respectivelyBack to article page