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Fig. 6 | Biotechnology for Biofuels

Fig. 6

From: A copper-controlled RNA interference system for reversible silencing of target genes in Trichoderma reesei

Fig. 6

Redesigned culture strategy to verify the functional role of fab1 in cellulase production. Two culture procedures (a) were designed for functional study of fab1 in T. reesei using the copper-responsive RNAi-mediated silencing system. The abundance of fab1 (b) and tcu1 (c) mRNAs was quantified by qRT-PCR in the appointed sampling times. Filter paper activity was measured when mycelia were transferred into Avicel induction media at indicated time (d). Extracellular fermentation liquor (20 μL) of P tcu1 –fab1KD strain cultured on 1% Avicel supplied with (e) or without (f) copper was analyzed by SDS-PAGE

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