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Table 1 Activity of CjGH5_4 enzymes against different polysaccharide substrates

From: In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions

Enzyme catalytic domains Substrate K m mg mL−1 kcat s−1 Specific activity µmol (min mg)−1
CjGH5D XyG < 0.025 30.3 ± 0.4 43.3 ± 1.9
CjGH5E XyG 0.020 ± 0.002 10.3 ± 0.1 15.1 ± 0.1
Hydroxyethylcellulose (HEC) ND ND 0.070 ± 0.004
Carboxymethylcellulose (CMC) ND ND 0.010 ± 0.002
CjGH5F XyG 0.040 ± 0.003 52.4 ± 0.8 74.8 ± 4.1
Hydroxyethylcellulose (HEC) ND ND 0.090 ± 0.003
  1. Assays conducted at pH 7.5 (CjGH5D and CjGH5E) or pH 7 (CjGH5F). Recombinant enzymes were incubated at 50 °C (CjGH5D and CjGH5F) or 55 °C (CjGH5E) with the different tested substrates
  2. ND not determined due to poor specific activity
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Biotechnology for Biofuels and Bioproducts

ISSN: 2731-3654

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