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Table 1 Activity of CjGH5_4 enzymes against different polysaccharide substrates

From: In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions

Enzyme catalytic domains

Substrate

K m mg mL−1

kcat s−1

Specific activity µmol (min mg)−1

CjGH5D

XyG

< 0.025

30.3 ± 0.4

43.3 ± 1.9

CjGH5E

XyG

0.020 ± 0.002

10.3 ± 0.1

15.1 ± 0.1

Hydroxyethylcellulose (HEC)

ND

ND

0.070 ± 0.004

Carboxymethylcellulose (CMC)

ND

ND

0.010 ± 0.002

CjGH5F

XyG

0.040 ± 0.003

52.4 ± 0.8

74.8 ± 4.1

Hydroxyethylcellulose (HEC)

ND

ND

0.090 ± 0.003

  1. Assays conducted at pH 7.5 (CjGH5D and CjGH5E) or pH 7 (CjGH5F). Recombinant enzymes were incubated at 50 °C (CjGH5D and CjGH5F) or 55 °C (CjGH5E) with the different tested substrates
  2. ND not determined due to poor specific activity