Enzyme catalytic domains
|
Substrate
|
K
m
mg mL−1
|
kcat s−1
|
Specific activity µmol (min mg)−1
|
---|
CjGH5D
|
XyG
|
< 0.025
|
30.3 ± 0.4
|
43.3 ± 1.9
|
CjGH5E
|
XyG
|
0.020 ± 0.002
|
10.3 ± 0.1
|
15.1 ± 0.1
|
Hydroxyethylcellulose (HEC)
|
ND
|
ND
|
0.070 ± 0.004
|
Carboxymethylcellulose (CMC)
|
ND
|
ND
|
0.010 ± 0.002
|
CjGH5F
|
XyG
|
0.040 ± 0.003
|
52.4 ± 0.8
|
74.8 ± 4.1
|
Hydroxyethylcellulose (HEC)
|
ND
|
ND
|
0.090 ± 0.003
|
- Assays conducted at pH 7.5 (CjGH5D and CjGH5E) or pH 7 (CjGH5F). Recombinant enzymes were incubated at 50 °C (CjGH5D and CjGH5F) or 55 °C (CjGH5E) with the different tested substrates
- ND not determined due to poor specific activity