|
Enzyme catalytic domains
|
Substrate
|
Km mM
|
kcat min−1
|
kcat/Km min−1 mM−1
|
|---|
|
CjGH5D
|
GGG-CNP
|
ND
|
ND
|
2.21 ± 0.05
|
|
GGGG-CNP
|
ND
|
ND
|
5.36 ± 0.07
|
|
XXXG-CNP
|
0.81 ± 0.10
|
281 ± 12
|
347 ± 45
|
|
XLLG-CNP
|
0.18 ± 0.02
|
162 ± 4
|
900 ± 103
|
|
CjGH5E
|
GGG-CNP
|
11.8 ± 0.6
|
191 ± 7
|
16.2 ± 1.0
|
|
GGGG-CNP
|
5.02 ± 0.35
|
180 ± 7
|
35.9 ± 2.8
|
|
XXXG-CNP
|
0.010 ± 0.001
|
254 ± 4
|
(25.4 ± 2.6) × 103
|
|
XLLG-CNP
|
0.010 ± 0.001
|
332 ± 9
|
(33.2 ± 3.4) × 103
|
|
CjGH5F
|
GGG-CNP
|
ND
|
ND
|
6.45 ± 0.30
|
|
GGGG-CNP
|
ND
|
ND
|
16.5 ± 1.0
|
|
XXXG-CNP
|
0.07 ± 0.01
|
169 ± 4
|
(2.41 ± 0.35) × 103
|
|
XLLG-CNP
|
0.030 ± 0.002
|
393 ± 8
|
(13.1 ± 0.9) × 103
|
- ND not determined due to limited availability of substrate
