Table 2 Kinetic parameters of CjGH5_4 enzymes for (xylo)gluco-oligosaccharide glycosides
Enzyme catalytic domains | Substrate | Km mM | kcat min−1 | kcat/Km min−1 mM−1 |
|---|---|---|---|---|
CjGH5D | GGG-CNP | ND | ND | 2.21 ± 0.05 |
GGGG-CNP | ND | ND | 5.36 ± 0.07 | |
XXXG-CNP | 0.81 ± 0.10 | 281 ± 12 | 347 ± 45 | |
XLLG-CNP | 0.18 ± 0.02 | 162 ± 4 | 900 ± 103 | |
CjGH5E | GGG-CNP | 11.8 ± 0.6 | 191 ± 7 | 16.2 ± 1.0 |
GGGG-CNP | 5.02 ± 0.35 | 180 ± 7 | 35.9 ± 2.8 | |
XXXG-CNP | 0.010 ± 0.001 | 254 ± 4 | (25.4 ± 2.6) × 103 | |
XLLG-CNP | 0.010 ± 0.001 | 332 ± 9 | (33.2 ± 3.4) × 103 | |
CjGH5F | GGG-CNP | ND | ND | 6.45 ± 0.30 |
GGGG-CNP | ND | ND | 16.5 ± 1.0 | |
XXXG-CNP | 0.07 ± 0.01 | 169 ± 4 | (2.41 ± 0.35) × 103 | |
XLLG-CNP | 0.030 ± 0.002 | 393 ± 8 | (13.1 ± 0.9) × 103 |