In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions

Table 2 Kinetic parameters of CjGH5_4 enzymes for (xylo)gluco-oligosaccharide glycosides

Enzyme catalytic domains	Substrate	K_m mM	k_cat min⁻¹	k_cat/K_m min⁻¹ mM⁻¹
CjGH5D	GGG-CNP	ND	ND	2.21 ± 0.05
	GGGG-CNP	ND	ND	5.36 ± 0.07
	XXXG-CNP	0.81 ± 0.10	281 ± 12	347 ± 45
	XLLG-CNP	0.18 ± 0.02	162 ± 4	900 ± 103
CjGH5E	GGG-CNP	11.8 ± 0.6	191 ± 7	16.2 ± 1.0
	GGGG-CNP	5.02 ± 0.35	180 ± 7	35.9 ± 2.8
	XXXG-CNP	0.010 ± 0.001	254 ± 4	(25.4 ± 2.6) × 10³
	XLLG-CNP	0.010 ± 0.001	332 ± 9	(33.2 ± 3.4) × 10³
CjGH5F	GGG-CNP	ND	ND	6.45 ± 0.30
	GGGG-CNP	ND	ND	16.5 ± 1.0
	XXXG-CNP	0.07 ± 0.01	169 ± 4	(2.41 ± 0.35) × 10³
	XLLG-CNP	0.030 ± 0.002	393 ± 8	(13.1 ± 0.9) × 10³