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Table 2 Kinetic parameters of CjGH5_4 enzymes for (xylo)gluco-oligosaccharide glycosides

From: In vitro and in vivo characterization of three Cellvibrio japonicus glycoside hydrolase family 5 members reveals potent xyloglucan backbone-cleaving functions

Enzyme catalytic domains Substrate Km mM kcat min−1 kcat/Km min−1 mM−1
CjGH5D GGG-CNP ND ND 2.21 ± 0.05
GGGG-CNP ND ND 5.36 ± 0.07
XXXG-CNP 0.81 ± 0.10 281 ± 12 347 ± 45
XLLG-CNP 0.18 ± 0.02 162 ± 4 900 ± 103
CjGH5E GGG-CNP 11.8 ± 0.6 191 ± 7 16.2 ± 1.0
GGGG-CNP 5.02 ± 0.35 180 ± 7 35.9 ± 2.8
XXXG-CNP 0.010 ± 0.001 254 ± 4 (25.4 ± 2.6) × 103
XLLG-CNP 0.010 ± 0.001 332 ± 9 (33.2 ± 3.4) × 103
CjGH5F GGG-CNP ND ND 6.45 ± 0.30
GGGG-CNP ND ND 16.5 ± 1.0
XXXG-CNP 0.07 ± 0.01 169 ± 4 (2.41 ± 0.35) × 103
XLLG-CNP 0.030 ± 0.002 393 ± 8 (13.1 ± 0.9) × 103
  1. ND not determined due to limited availability of substrate