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Table 1 Primers used for PCR-DGGE and thermal denaturation conditions of the primers

From: The structural and functional contributions of β-glucosidase-producing microbial communities to cellulose degradation in composting

Primer Sequences (5′–3′) Thermal conditions
GH1 family gene (bacteria)
 GH1BF CCT ACC AGA TYC ARG G  
 GH1BR GAG GAA GRT CCC ART G 95 °C, 30 s, one cycle, 95 °C for 5 s, 50 °C for 30 s, 72 °C for 60 s, 45 cycles
 GH1BF-GC CCT ACC AGA TYG ARG GCGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG G  
GH3 family gene (fungi)
 GH3EF GGT GGT CGC RRY TGG GA  
 GH3ER CCA GGC ATC GGW CAT RTC 95 °C, 30 s, one cycle, 95 °C for 5 s, 56 °C for 30 s, 72 °C for 60 s, 45 cycles
 GH3ER-GC CGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGG TGG TCG CRR YTG GGA  
  1. Modified bases: Y = CT, R = AG, W = TA
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