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Table 1 Primers used for PCR-DGGE and thermal denaturation conditions of the primers

From: The structural and functional contributions of β-glucosidase-producing microbial communities to cellulose degradation in composting

Primer

Sequences (5′–3′)

Thermal conditions

GH1 family gene (bacteria)

 GH1BF

CCT ACC AGA TYC ARG G

 

 GH1BR

GAG GAA GRT CCC ART G

95 °C, 30 s, one cycle, 95 °C for 5 s, 50 °C for 30 s, 72 °C for 60 s, 45 cycles

 GH1BF-GC

CCT ACC AGA TYG ARG GCGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG G

 

GH3 family gene (fungi)

 GH3EF

GGT GGT CGC RRY TGG GA

 

 GH3ER

CCA GGC ATC GGW CAT RTC

95 °C, 30 s, one cycle, 95 °C for 5 s, 56 °C for 30 s, 72 °C for 60 s, 45 cycles

 GH3ER-GC

CGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGG TGG TCG CRR YTG GGA

 
  1. Modified bases: Y = CT, R = AG, W = TA