Primer | Sequences (5′–3′) | Thermal conditions |
---|---|---|
GH1 family gene (bacteria) | ||
GH1BF | CCT ACC AGA TYC ARG G | |
GH1BR | GAG GAA GRT CCC ART G | 95 °C, 30 s, one cycle, 95 °C for 5 s, 50 °C for 30 s, 72 °C for 60 s, 45 cycles |
GH1BF-GC | CCT ACC AGA TYG ARG GCGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG G | |
GH3 family gene (fungi) | ||
GH3EF | GGT GGT CGC RRY TGG GA | |
GH3ER | CCA GGC ATC GGW CAT RTC | 95 °C, 30 s, one cycle, 95 °C for 5 s, 56 °C for 30 s, 72 °C for 60 s, 45 cycles |
GH3ER-GC | CGC CCG CCG CGC GCG GCG GGC GGG GCG GGG GCA CGG GGG TGG TCG CRR YTG GGA |