Table 3 Universal and specific primers of β-glucosidase genes for real-time quantitative PCR
Primer | Sequences (5′–3′) | Primer | Sequences (5′–3′) |
|---|---|---|---|
GH1 family genes (bacteria) | Microbacterium | ||
GH1BF | CCT ACC AGA TYC ARG G | GH1B-d1F | ACGACCATTGACCAGACGGGAGT |
GH1BR | GAG GAA GRT CCC ART G | GH1B-d1R | GAAGGAGCGGCTGACGAGGAC |
Bacillus sp. | GH3 family genes (fungi) | ||
GH1B-a1F | AGATTGAAGGAGCAAGGAATGAGG | GH3EF | GGT GGT CGC RRY TGG GA |
GH1B-a1R | AGGAAGGTCCCAGTGGTAGAGC | GH3ER | CCA GGC ATC GGW CAT RTC |
Devosia sp. | Aspergillus niger | ||
GH1B-b1F | ACCAGAACGAAGGCGGGCAGAC | GH3E-d3F | GGCAAGGCTATGGGTCAG |
GH1B-b1R | CCAGTCATAGAGCGTGGAGTAG | GH3E-d3R | CCAGCGGAAATGCTCTAAT |
Streptomyces sp. | Penicillium | ||
GH1B-c1F | CGAGCATACACTGGGCAACA | GH3E-e3F | TATGGGTGGTGATGTGAAT |
GH1B-c1R | GAAGGTTVVAATGGCACAGCGT | GH3E-e3R | CAAGTGGCTGGTTGAGTT |