Table 1 Saccharomyces cerevisiae strains used in this study

CEN.PK 113-7D

MATa URA3 HIS3 LEU2 TRP1 MAL2-8c SUC2

[36]

CEN.PK 113-5D

MATa ura3-52 HIS3 LEU2 TRP1 MAL2-8c SUC2

[36]

CEN.PK102-12A

MATa ura3-52 his3-D1 leu2-3,112 TRP1 MAL2-8c SUC2

[36]

IMX080

CEN.PK102-12A glk1::SpHis5, hxk1::KlLEU2

[75]

IMX581

CEN.PK113-5D can1::cas9-natNT2

[44]

IMX486

IMX080 gal1::cas9-amdSYM

This study

IMX604

IMX486 ura3-52 gre3::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2

This study

IMX658

IMX604 ura3-52 gal80::(pTPI-AraA-tCYC1)*9, pPYK1-AraB-tPGI1, pPGK1-AraD-tTDH3

This study

IMX660

IMX658 hxk2::KlURA3

This study

IMX728

IMX658 hxk2::PcaraT

This study

IMX844

IMX660 gal2::KanMX

This study

IMX869

IMX728 gal2::KanMX

This study

IMX918

IMX581 gre3::pTDH3-RPE1, pPGK1-TKL1, pTEF1-TAL1, pPGI1-NQM1, pTPI1-RKI1, pPYK1-TKL2

This study

IMX928

IMX918 gal80::(pTPI-AraA-tCYC1)*9, pPYK-AraB-tPGI1, pPGK-AraD-tTDH3

This study

IMX929

IMX918 gal80::(pTPI-AraA-tCYC1)*9, pPYK-AraB-tPGI1, pPGK-AraD-tTDH3, pUDE348

This study

IMX1504

IMX928, gal2Δ, pUDR245

This study

IMX1505

IMX928 gal2::pADH1-Pc13g04640-tPMA1 (from pPWT111), pUDR245

This study

IMX1506

IMX928 gal2::pADH1-Pc13g08230-tPMA1 (from pPWT113), pUDR245

This study

IMX1507

IMX928 gal2::pADH1-Pc16g05670-tPMA1 (from pPWT116), pUDR245

This study

IMX1508

IMX928 gal2::pADH1-Pc20g01790-tPMA1 (PcaraT) (from pPWT118), pUDR246

This study

IMX1509

IMX928 gal2::pADH1-Pc22g14520-tPMA1 (from pPWT123), pUDR245

This study

DS68616

MATa, ura3-52, leu2-112, gre3::loxP, loxP-pTPI-TAL1, loxP-pTPI-RKI1, loxP-pTPI-TKL1, loxP-pTPI-RPE1, leu2::pADH1-XKS1-tCYC1-LEU2, ura3::URA3-pTPI1-XylA-tCYC1

DSM, The Netherlands

DS68625

DS68616 his3::loxP, hxt2::loxP-kanMX-loxP, hxt367::loxP-hphMX-loxP, hxt145::loxP-natMX-loxP, gal2::loxP-zeoMX-loxP

[45]

DS68625-PcaraT

DS68625, pRS313-PcaraT

This study

DS68625-GAL2

DS68625, pRS313-GAL2

This study

DS68625-mcs

DS68625, pRS313-mcs (empty)

This study