Table 2 Plasmids used in this study

p414-TEF1p-Cas9-CYC1t

CEN6/ARS4 ampR pTEF1-cas9-tCYC1

[76]

pUG-amdSYM

Template for amdSYM marker

[38]

pUG-72

Template for KlURA3 marker

[77]

pUG6

Template for KanMX marker

[78]

pUDE327

2 μm, KlURA3, pSNR52-gRNA.HXK2.Y

[79]

pUDE335

2 μm, KlURA3, pSNR52-gRNA.GRE3.Y

[50]

pUDE348

2 μm, KlURA3, pSNR52-gRNA.GAL80.Y

This study

pUDR246

2 μm, KlURA3, pSNR52-gRNA.GAL2.Y pSNR52-gRNA.GAL2.Y

This study

pUDR245

2 μm, KlURA3, pSNR52-gRNA.GAL2.Y pSNR52-gRNA.GAL2.Y

This study

pMEL10

pSNR52-gRNA.CAN1.Y-tSUP4

[44]

pROS10

2 μm, KlURA3, pSNR52-gRNA.CAN1.Y pSNR52-gRNA.ADE2.Y

[44]

pUD344

pJET1.2Blunt TagA-pPGI1-NQM1-TagB

[50]

pUD345

pJET1.2Blunt TagB-pTPI1-RKI1-TagC

[50]

pUD346

pJET1.2Blunt TagC-pPYK1-TKL2-TagF

[50]

pUD347

pJET1.2BluntTagG-pTDH3-RPE1-TagH

[50]

pUD348

pJET1.2Blunt TagH-pPGK1-TKL1-TagI

[50]

pUD349

pJET1.2Blunt TagI-pTEF1-TAL1-TagA

[50]

pUD405

pJET1.2Blunt GAL2 flanked KanMX

This study

pPWT111

ampR KanMX, amdSYM, pADH1-Pc13g04640-tPMA1

This study

pPWT113

ampR KanMX, amdSYM, pADH1-Pc13g08230-tPMA1

This study

pPWT116

ampR KanMX, amdSYM, pADH1-Pc16g05670-tPMA1

This study

pPWT118

ampR KanMX, amdSYM, pADH1-Pc20g01790 (PcaraT)-tPMA1

This study

pPWT123

ampR KanMX, amdSYM, pADH1-Pc22g14520-tPMA1

This study

pUD354

pMK-RQ-pTPI1-araA-tADH3

This study

pUD355

pMK-RQ-pPYK1-araB-tPGI1

This study

pUD356

pMK-RQ-pPGK1-araD-tTDH3

This study

pRS313-mcs

CEN6, ARSH4, HIS3-pHXT7, tHXT7

[45]

pRS313-PcaraT

CEN6, ARSH4, HIS3, ampR, pHXT7-PcaraT-tHXT7

This study

pRS313-GAL2

CEN6, ARSH4, HIS3, ampR, pHXT7-GAL2-tHXT7

This study